Influences of pilates training on postural balance in martial arts students / Influências do treinamento de pilates sobre o equilíbrio postural em alunos de artes marciais / Influencia del entrenamiento de pilates en el equilibrio postural de estudiantes de artes marciales

ABSTRACT Introduction: Physical stability is extremely important for student-athletes of martial arts, and it is believed that the practice of Pilates can generate benefits in this aspect. Objective: Analyze the influences of Pilates training on postural balance in martial arts students during daily training. Methods: Using the control experiment verification method, this study selected 60 martial arts students and athletes to perform the control experiment. They were randomly divided into the experimental group and the control group. In addition to the traditional daily martial arts training protocol, the experimental group underwent Pilates training, while the control group performed only daily training. Results: In the experimental Pilates training group, vertical balance time increased from 791.25 ± 158.48 ms to 695.64 ± 208.08 ms, and left-right laterality balance time increased from 1,861.04 ± 202.96 ms to 1,994.70 ± 189.67 ms, and anteroposterior balance time increased from 1243.24 ± 475.07 ms to 1419.52 ± 331.62 ms. The balance score increased from 53.16 ± 3.94 to 63.83 ± 3.12. Conclusion: Pilates training improved postural balance in martial arts students. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução: A estabilidade física é extremamente importante para os estudantes atletas de artes marciais, acredita-se que a prática de pilates possa gerar benefícios neste aspecto. Objetivo: Analisar as influências do treinamento de Pilates sobre o equilíbrio postural em estudantes de artes marciais durante o treinamento diário. Métodos: Usando o método de verificação do experimento de controle, este trabalho selecionou 60 alunos e atletas de artes marciais escolares para realizar o experimento de controle. Eles foram divididos aleatoriamente no grupo experimental e no grupo de controle. Além do protocolo tradicional de treinamento diário de artes marciais, o grupo experimental foi submetido ao treinamento Pilates, enquanto o grupo de controle realizou apenas o treinamento diário. Resultados: No grupo experimental de treinamento Pilates, o tempo de equilíbrio vertical aumentou de 791,25 ± 158,48 ms para 695,64 ± 208,08 ms, o tempo de equilíbrio de lateralidade esquerda e direita aumentou de 1.861,04 ± 202,96 ms para 1.994,70 ± 189,67 ms, e o tempo de equilíbrio antero-posterior elevou-se de 1243,24 ± 475,07 ms para 1419,52 ± 331,62 ms. A pontuação de equilíbrio aumentou de 53,16 ± 3,94 para 63,83 ± 3,12. Conclusão: O treinamento do Pilates demonstrou melhorar o equilíbrio postural dos estudantes de artes marciais. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción: La estabilidad física es extremadamente importante para los estudiantes atletas de artes marciales, se cree que la práctica de Pilates puede generar beneficios en este aspecto. Objetivo: Analizar las influencias del entrenamiento de Pilates sobre el equilibrio postural en estudiantes de artes marciales durante el entrenamiento diario. Métodos: Utilizando el método de verificación del experimento de control, este estudio seleccionó a 60 estudiantes y atletas de artes marciales para realizar el experimento de control. Se dividieron aleatoriamente en el grupo experimental y en el grupo de control. Además del protocolo de entrenamiento diario tradicional de artes marciales, el grupo experimental se sometió a entrenamiento de Pilates, mientras que el grupo de control sólo realizó entrenamiento diario. Resultados: En el grupo experimental de entrenamiento de Pilates, el tiempo de equilibrio vertical aumentó de 791,25 ± 158,48 ms a 695,64 ± 208,08 ms, el tiempo de equilibrio de lateralidad izquierda y derecha aumentó de 1.861,04 ± 202,96 ms a 1.994,70 ± 189,67 ms, y el tiempo de equilibrio anteroposterior aumentó de 1.243,24 ± 475,07 ms a 1.419,52 ± 331,62 ms. La puntuación de equilibrio aumentó de 53,16 ± 3,94 a 63,83 ± 3,12. Conclusión: El entrenamiento con Pilates demostró mejorar el equilibrio postural en estudiantes de artes marciales. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Analysis of monitoring results of novel coronavirus IgM and IgG antibody levels in apheresis platelets donors and impact on blood safety / 中国输血杂志

【Objective】 To monitor the positive rates of IgM and IgG antibodies and the changes in S/CO values over time in voluntary blood donors infected with SARS-CoV-2 after recovery, in order to provide theoretical basis and data support for ensuring clinical blood safety. 【Methods】 A total of 54 platelet donors who met the inclusion criteria were selected for this study, and 359 blood samples (plasma) from T1 to T7 (at 7 time points, spanning 14 to 15 weeks) were continuously monitored for SARS-CoV-2 IgM and IgG antibodies using fully automated chemi-luminescence method. 【Results】 Among 359 blood samples (plasma) from 54 donors, 349 were with valid test results.Eleven donors were tested positive for IgM, with a positive rate of 20.37%, and IgM antibody S/CO value gradually increased during T1-T3, but gradually decreased during T4-T7. Fifty-four donors were tested positive for IgG, with the positive rate of 100%, and the S/CO value of IgG antibody gradually decreased over time. During the period of T1 -T7, there was no significant difference in SARS-CoV-2 IgG antibody S/CO value between gender (male/female) or age group (18-39 years old /40-60 years old). 【Conclusion】 The positive rate of SARS-CoV-2 IgG antibody in platelet donors after recovery from SARS-CoV-2 infection was 100% during 2-14 weeks, and the IgG S/CO value gradually decreases over time. The long-term dynamic changes of SARS-CoV-2 serologically specific IgG and IgM antibodies in blood donors are worthy of further study.

Factors influencing hemolysis rate of leukocyte-reduced red blood cells at the end of preservation period in blood donors / 中国输血杂志

【Objective】 To compare the current standards and explore the influencing factors for hemolysis rate of leukocyte-reduced red blood cells at the end of the preservation period, in order to formulate reasonable internal control indicators. 【Methods】 A retrospective analysis was performed on hemolysis rate of 427 samples of leukocyte-reduced red blood cells at the end of the preservation period in Nanning Blood Center from 2015 to 2022. Compared with the current standard for hemolysis rate at the end of the preservation period (GB 18469-2012 Quality Requirements for Whole Blood and Component Blood), the differences were analyzed, and the factors influncing the hemolysis rate were analyzed in terms of different blood donor groups. 【Results】 1) Among the 427 samples, the hemolysis rate of 418 (97.89%) did not exceed 0.4%, all lower than 0.8%; 2)the hemolysis rate of the male group was higher than that of the female group; 3) the hemolysis rate of the 18-29 years old group was lower than that of the 30-39 year old group and the 40-60 year old group, with statistically significant difference; 4) in terms of occupation, the hemolysis rate of students was the lowest, and the differences between groups were statistically significant; 5) no statistical significance was found in ethnicity and blood type. 【Conclusion】 Statistics indicated that gender, age, blood donation volume and occupation of blood donors were the influencing factors of hemolysis rate. The current standard is obviously higher in the qualified range of blood quality control in Nanning. It is advisable to formulate a reasonable quality control strategy with internal control index of hemolysis rate set <0.4%, which is conducive to making accurate evaluation of internal quality control and ensuring blood safety.

Diagnostic value of fractional exhaled nitric oxide combined with small airway function test for chronic cough / 中国基层医药

Objective:To investigate the value of fractional exhaled nitric oxide (FeNO) combined with small airway function test to replace bronchial provocation test and induced sputum test in differentiating cough variant asthma (CVA) from eosinophilic bronchitis (EB).Methods:The clinical data of 105 patients with chronic cough admitted to The Third People's Hospital of Hubei, Jianghan University from January 2018 to December 2021 were retrospectively analyzed. These patients consisted of 40 patients with CVA (CVA group), 25 patients with EB (EB group), and 40 patients with other chronic coughs (other chronic cough group). FeNO and lung function were compared between groups. The value of FeNO, small airway function, and their combination in differentiating CVA from EB were analyzed using the receiver operating characteristic curves.Results:FeNO level was the highest in the CVA group [33.0 (30.0, 37.8) ppb], followed by the EB group [28.0 (25.5, 32.0) ppb], and the lowest in other chronic cough group [13.0 (11.0, 15.0) ppb]. There was significant difference in FeNO level between groups ( H value = 79.00, P < 0.05). There were no significant differences in forced vital capacity (FVC), forced expiratory volume in 1 second (FEV 1), FEV 1/FVC, peak expiratory flow (PEF) between groups (all P > 0.05). Maximal mid-expiratory flow (MMEF) [74 (66.0, 77.4) in the CVA group, 80 (79.0, 83.3) in the EB group, 88.0 (86.4, 90.0) in other chronic coughs group], FEF25 (%) [70.0 (60.3, 75.1) in the CVA group, 78.0 (74.1, 85.0) in the EB group, 81.7 (78.9, 86.3) in other chronic coughs group], FEF50 (%) [75.2 (67.1, 80.8) in the CVA group, 80.6 (75.7, 85.9) in the EB group, 89.4 (87.0, 90.5) in other chronic coughs group], FEF75 (%) [76.4 (68.7, 85.8) in the CVA group, 80.9 (77.4, 89.7) in the EB group, 90.8 (87.2, 94.2) in other chronic coughs group] were significantly lower in the CVA group than those in other chronic coughs group. With the exception of FEF25 (%), MMEF (%), FEF50 (%), and FEF75 (%) were significantly lower in the EB group compared with other chronic coughs group. MMEF (%) and FEF25 (%) in the CVA group were significantly lower compared with the EB group. There were significant differences in MMEF (%), FEF50 (%), and FEF75 (%) between groups ( H = 62.82, 47.04, 47.41, 49.11, all P < 0.01). There were significant differences in FEF50 (%) and FEF75 (%) between CVA and EB groups (both P > 0.05). In binary logistic regression equation, FeNO and MMEF (%) were important indexes to distinguish CVA from EB ( P < 0.05). Bronchial provocation test and induced sputum test were used as the gold standard to distinguish CVA from EB. When FeNO and MMEF (%) were used separately to distinguish CVA from EB, the optimal threshold value was 30.0 ppb and 77.7 respectively, the area under the receiver operating characteristic curve was 0.77 and 0.82 respectively, the diagnostic sensitivity was 70% and 77.5% respectively, and the diagnostic specificity was 72% and 88% respectively. When FeNO and MMEF (%) were used in combination to distinguish CVA from EB, the area under the receiver operating characteristic curve was 0.89, and the diagnostic sensitivity and specificity was 75% and 96% respectively. Conclusion:FeNO and MMEF (%) can be used to distinguish CVA from EB. FeNO combined with MMEF (%) has a higher value in distinguishing CVA from EB than FeNO and MMEF alone.

Guideline for postoperative rehabilitation treatment following vertebral augmentation for osteoporotic vertebral compression fracture (version 2022) / 中华创伤杂志

Osteoporotic vertebral compression fracture (OVCF) can lead to lower back pain and may be even accompanied by scoliosis, neurological dysfunction and other complications, which will affect the daily activities and life quality of patients. Vertebral augmentation is an effective treatment method for OVCF, but it cannot correct unbalance of bone metabolism or improve the osteoporotic status, causing complications like lower back pain, limited spinal activities and vertebral refracture. The post-operative systematic and standardized rehabilitation treatments can improve curative effect and therapeutic efficacy of anti-osteoporosis, reduce risk of vertebral refracture, increase patient compliance and improve quality of life. Since there still lack relevant clinical treatment guidelines for postoperative rehabilitation treatments following vertebral augmentation for OVCF, the current treatments are varied with uneven therapeutic effect. In order to standardize the postoperative rehabilitation treatment, the Spine Trauma Group of the Orthopedic Branch of Chinese Medical Doctor Association organized relevant experts to refer to relevant literature and develop the "Guideline for postoperative rehabilitation treatment following vertebral augmentation for osteoporotic vertebral compression fracture (2022 version)" based on the clinical guidelines published by the American Academy of Orthopedic Surgeons (AAOS) as well as on the principles of scientificity, practicality and advancement. The guideline provided evidence-based recommendations on 10 important issues related to postoperative rehabilitation treatments of OVCF.

Acupuncture treatment for knee osteoarthritis: an overview of systematic reviews / 国际中医中药杂志

Objective:To revaluate the systematic review/meta-analysis on efficacy and safety of acupuncture treatment for knee osteoarthritis.Methods:We searched CNKI, VIP, WangFang, CBM, PubMed, Cochrane Library databases, Embase, from inception to December 15, 2020, to retrieve systematic reviews and/or meta analyses on the treatment of knee osteoarthritis with acupuncture and moxibustion. Two researchers independently screened the literature and extracted data, used the AMSTAR2 scale to evaluate the methodological quality, and used the GRADE system to grade the quality of evidence.Results:A total of 11 systematic evaluations were included, including 44 outcomes. Methodological evaluation showed that all 11 literatures were of very low quality. The GRADE system evaluation showed that there were 13 intermediate evidences, 12 low evidences, and 19 very low evidences.Conclusion:The results of the existing systematic reviews show that acupuncture and moxibustion have a certain efficacy in the treatment of knee osteoarthritis, but the methodology and evidence level of the included studies are low, so this conclusion needs to be verified by further high-quality studies.

Research progress of triple therapy in chronic obstructive pulmonary disease / 中国医师杂志

Chronic obstructive pulmonary disease (COPD) is a chronic airway disease, which is mainly characterized by persistent respiratory symptoms and irreversible airflow limitation, with high prevalence and high mortality. Exacerbations of COPD will lead to deterioration of lung function and disease progression, which is the focus of COPD management. Pharmacological therapy for COPD is used to improve symptoms, lung function and health status, prevent and treat exacerbation. Triple inhaled therapy for COPD includes long-acting muscarinic antagonist (LAMA), long-acting β 2-agonist (LABA) and inhaled corticosteroid (ICS). This article will review the research progress of triple inhaled therapy for COPD, and provide a reference for the clinical management of COPD.

Matrix metalloproteinase regulates natural killer cells function in methicilin-resistant staphylococcus aureus sepsis / 中华急诊医学杂志

Objective:To investigate the characteristics of natural killer (NK) cell subsets and function in methicillin-resistant staphylococcus aureus (MRSA) sepsis, and to assess the influence of matrix metalloproteinase (MMP) to NK cell function in MRSA sepsis patients.Methods:Twenty-one MRSA sepsis patients who were hospitalized in our department between January 2017 and June 2018 were enrolled. Eleven healthy individuals were served as healthy controls. Peripheral blood mononuclear cells (PBMCs) were isolated. NK cell subsets were investigated by flow cytometry. NK cell function was assessed by measuring CD107a, CD69, and CD16 expression in co-culture system between PBMCs and different target cells. MMP mRNA was semi-quantified by real-time PCR in purified NK cells. The influence of NK cell function was assessed by measuring CD107a expression in co-culture system between NK cells with MMP inhibitor stimulation and target cells.Results:There was no significant difference of total NK cell percentage between healthy controls and MRSA sepsis patients ( P>0.05). CD56brightCD16 -NK [(5.36±1.02)% vs (4.30±0.89)%] and CD56 -CD16 +NK [(24.04±2.92)% vs (9.70±1.54)%] percentage was elevated ( P<0.05), while CD56dimCD16 +NK percentage [(71.22±13.03)% vs (87.64±7.05)%, P<0.01] was reduced in MRSA sepsis. NK cells recognized and killed target cells via different receptors upon activation. CD107a [(33.55±3.84)% vs (25.34±6.20)%] and CD69 percentage [(14.96±1.47)% vs(18.80±1.49)%] was decreased ( P<0.0001), while CD16 MFI was increased [(247.1±50.31) vs (189.4±57.54), P<0.01] in MRSA sepsis patients in comparison with healthy controls. MMP-1/2/3/9 mRNA relative levels were elevated in purified NK cells from MRSA sepsis patients ( P<0.01). Inhibition of MMP in NK cells from MRSA sepsis patients promoted CD107a percentage [(33.67±8.03)% vs (25.87±6.23)%, P=0.018]. Conclusions:NK cell subsets imbalance and exhaustion is existed in MRSA sepsis, which might be due to the MMP-induced down-regulation of antibody-dependent cell-mediated cytotoxicity.

Serum microRNA-381: A Potential Marker for Early Diagnosis of Gastric Cancer

PURPOSE: The purpose of this study was to explore the potential early diagnostic value of serum microRNA-381(miRNA-381) in patients with gastric cancer (GC). MATERIALS AND METHODS: Patients with advanced gastric cancer (AGC) and early gastric cancer (EGC), as well as healthy individuals, were enrolled in this study. Expression of miRNA-381 in serum was detected using real-time quantitative PCR. Electrochemiluminescence analysis was used to investigate the expression of classic tumor markers, including carbohydrate antigen (CA) 199, CA724, and carcinoembryonic antigen. Finally, receiver operating characteristic curve and Kaplan-Meier analysis were used to determine the value of miRNA-381 in clinical diagnosis of GC. RESULTS: miRNA-381 was differentially expressed among the study groups. AUC analysis showed that the sensitivity and specificity of serum miRNA-381 in the diagnosis of GC were superior to those of other tumor markers. Furthermore, low levels of miRNA-381 expression were positively correlated with lymph node metastasis and AGC. Finally, Kaplan-Meier survival analysis showed that down-regulation of miRNA-381 was associated with lymph node metastasis and the development of GC. CONCLUSION: miRNA381, which was down-regulated in GC, might be a novel early diagnosis marker for patients with GC.

Effects of low-intensity pulsed ultrasound and pioglitazone on chondrocytes in osteoarthritis / 中华物理医学与康复杂志

Objective To investigate any protective effect of low-intensity pulsed ultrasound (LIPUS) and pioglitazone on chondrocytes in osteoarthritic patients using the pathway from peroxisome proliferator-activated γreceptor (PPARγ) to nuclear factor kappa B (NF-κB) to inducible nitric oxide synthase (iNOS).Methods Normal chondrocytes of 24 healthy adult New Zealand white rabbits were extracted and divided into a normal group,a lipopolysaccharide (LPS) group,a LIPUS group (LPS+LIPUS) and a pioglitazone group (LPS+pioglitazone),each of 6 using a random number table.Each group was given the intervention their names implies.The levels of tumor necrosis factor-α (TNF-α),leptin (LEP) and nitric oxide (NO) in the chondrocytes were detected using enzyme-linked immune sorbent assays.The expression of type Ⅱ collagen (COL2) in the chondrocytes of each groups was detected using immunocytochemistry and fluorescent staining.The mRNA and protein expressions of PPARγ,NF-κB and iNOS were detected using reverse transcription polymerase chain reactions and western blotting respectively.Results Compared with the LPS group,the average level of TNF-α,LEP and NO in the LIPUS and pioglitazone groups was significantly lower,with the levels in the pioglitazone group significantly lower than in the LIPUS group.Compared with the LPS group,COL2 expression in the LIPUS group was significantly greater.The mRNA and protein expressions of PPARγ in the chondrocytes in the LIPUS and pioglitazone groups were significantly higher than those in the LPS group.Compared with the LPS group,the mRNA and protein expressions of NF-κB and iNOS in the pioglitazone and LIPUS groups were significantly lower,with the pioglitazone group's levels significantly below those of the LIPUS group.Conclusion LIPUS and pioglitazone may promote anti-inflammatory action and COL2 synthesis in chondrocytes through the PPARγ/ NF-κB/iNOS pathway and play a protective role,at least in rabbits.

Pelvic peritoneum reconstuction with barbed suture combined with irrigation and negative pressure drainage in laparoscopic rectal anterior resection for rectal cancer / 中华普通外科杂志

Objective To evaluate pelvic peritoneum reconstruction with barbed suture and irrigation and negative pressure drainage in laparoscopy rectal anterior resection for locally advanced rectal cancer.Methods From 2014 to 2018 159 locally advanced rectal cancer patients treated by laparoscopy were derided into study group (n =76),and those received gravity drainage without pelvic peritoneum reconstruction (n =83).Results There were not significant difference in the operation time between the two groups [(165 ± 18) min vs.(160 ± 17) min,t =0.098,P =0.069].There were significant differences in postoperative drainage tube indwelling duration [(5.7 ± 2.4) d vs.(7.4 ± 3.7) d,t =3.309,P =0.001],intraabdominal infection (7％ vs.17％,x2 =3.991,P =0.046) and anastomotic leakage (4％ vs.13％,x/2 =4.280,P =0.039).There was no significant difference in anastomotic stenosis (5％ vs.10％,x2 =1.088,P =0.297) and intestinal obstruction (9％ vs.11％,x2 =0.117,P =0.732) between the two groups.Conclusion It is simple,safe and reliable to perform pelvic peritoneal reconstruction using barbed suture,combined with irrigation and negative pressure drainage,it can decrease drainage tube indwelling duration,reduce the incidence of intraabdominal infection and anastomotic leakage.

Effect of continuous negative pressure drainage with intermittent irrigation on surgical site infection after laparoscopic extralevator abdominoperineal excison / 中华胃肠外科杂志

<p><b>OBJECTIVE</b>To explore the effect of continuous negative pressure drainage with intermittent irrigation on surgical site infection (SSI) after laparoscopic extralevator abdominoperineal excison (ELAPE).</p><p><b>METHODS</b>Clinical data of 28 rectal cancer patients who underwent continuous negative pressure drainage with intermittent irrigation following laparoscopic ELAPE (negative irrigation group) at our department from March 2016 to August 2017 were analyzed retrospectively. At the same time, 32 rectal cancer patients who underwent laparoscopic ELAPE and simple presacral drainage from January 2014 to February 2016 were included as controls (simple drainage group). Self-made double cannula: one silicon rubber drainage tube was used; 3 side holes were cut at the front end with 1-2 cm interval; tube was ranked intermittently and oppositely; a small hole was cut in the middle of rear; the infusion tube was placed through the small hole to the front side of the drainage tube (to rinse when the drainage was turbid). The placement and use of self-made double cannula: it was placed in the presacral space and was drawn from the medial to the sciatic tubercle, then was connected to drainage bag for 24 hours; when no blood was observed, the drainage tube was connected to negative pressure drainage ball, keeping negative pressure status. The development of SSI within 30 days postoperatively and other perioperative parameters were compared between the two groups.</p><p><b>RESULTS</b>There were no statistically significant differences in baseline data between two groups (all P>0.05). Incidence of SSI in negative irrigation group was significantly lower than that in simple drainage group [14.3% (4/28) vs. 43.8% (14/32), χ=6.173, P=0.013]. Additionally, a shorter postoperative hospital stay was observed in negative irrigation group [(9.8±1.5) days vs. (11.4±2.6) days, t=2.918, P=0.005]. Besides, other perioperative parameters, including operative time, intraoperative blood loss, time to removal of drainage tube, etc were not significantly different between two groups (all P>0.05). After adjusting to confounders, multivariate analysis showed that negative pressure drainage was an independent protective factor for SSI following laparoscopic ELAPE (OR=0.214, 95%CI:0.060-0.762, P=0.002).</p><p><b>CONCLUSION</b>Continuous negative pressure drainage with intermittent irrigation can effectively decrease the incidence of SSI following laparoscopic ELAPE, and is safe and simple.</p>

Risk factors for infectious complications after hepatectomy in patients with intrahepatic bile duct stones / 临床肝胆病杂志

Objective To investigate the risk factors for infectious complications after hepatectomy in patients with intrahepatic bile duct stones.Methods A retrospective analysis was performed for the clinical data of 168 patients with intrahepatic bile duct stones who underwent hepatectomy in The Fifth People's Hospital of Ji'nan from January 2010 to December 2016.A univariate analysis was performed for potential risk factors for infectious complications after hepatectomy,and the chi-square test was used for comparison of categorical data between groups.A multivariate logistic regression analysis was performed for the factors with statistical significance.Results Of all patients,31 (18.5％) experienced infectious complications after surgery.No patient underwent reoperation and all patients were cured.The univariate analysis showed that age,preoperative albumin level,biliary cirrhosis,interval between surgery and acute cholangitis (ISAC),extent of hepatectomy,and choledochoenterostomy were influencing factors for postoperative infectious complications (x2 =5.407,4.263,11.633,6.023,5.000,and 5.815,all P ＜ 0.05).The multivariate analysis showed that biliary cirrhosis (odds ratio [OR] =3.028,95％ confidence interval [CI]:1.791-5.121,P =0.002),ISAC ＜ 4 weeks (OR =1.539,95 ％ CI:1.010-2.345,P =0.046),and choledochoenterostomy (OR =2.389,95 ％ CI:1.261-4.527,P =0.028) were independent risk factors for infectious complications after hepatectomy in patients with intrahepatic bile duct stones.Conclusion Patients with intrahepatic bile duct stones,particularly those with biliary cirrhosis,ISAC ＜ 4 weeks,and choledochoenterostomy,have a high risk of developing infectious complications after hepatectomy.Control and improvement of these factors may help with the early prevention of postoperative infectious complications.

Risk factors for infectious complications after hepatectomy in patients with intrahepatic bile duct stones / 临床肝胆病杂志

Objective To investigate the risk factors for infectious complications after hepatectomy in patients with intrahepatic bile duct stones.Methods A retrospective analysis was performed for the clinical data of 168 patients with intrahepatic bile duct stones who underwent hepatectomy in The Fifth People's Hospital of Ji'nan from January 2010 to December 2016.A univariate analysis was performed for potential risk factors for infectious complications after hepatectomy,and the chi-square test was used for comparison of categorical data between groups.A multivariate logistic regression analysis was performed for the factors with statistical significance.Results Of all patients,31 (18.5％) experienced infectious complications after surgery.No patient underwent reoperation and all patients were cured.The univariate analysis showed that age,preoperative albumin level,biliary cirrhosis,interval between surgery and acute cholangitis (ISAC),extent of hepatectomy,and choledochoenterostomy were influencing factors for postoperative infectious complications (x2 =5.407,4.263,11.633,6.023,5.000,and 5.815,all P ＜ 0.05).The multivariate analysis showed that biliary cirrhosis (odds ratio [OR] =3.028,95％ confidence interval [CI]:1.791-5.121,P =0.002),ISAC ＜ 4 weeks (OR =1.539,95 ％ CI:1.010-2.345,P =0.046),and choledochoenterostomy (OR =2.389,95 ％ CI:1.261-4.527,P =0.028) were independent risk factors for infectious complications after hepatectomy in patients with intrahepatic bile duct stones.Conclusion Patients with intrahepatic bile duct stones,particularly those with biliary cirrhosis,ISAC ＜ 4 weeks,and choledochoenterostomy,have a high risk of developing infectious complications after hepatectomy.Control and improvement of these factors may help with the early prevention of postoperative infectious complications.

Neoadjuvant therapy of regional intra-arterial chemoembolization combined systemic vein chemotherapy for locally advanced gastric cancer / 中华普通外科杂志

Objective To investigate the efficacy and safety of preoperative regional intra-arterial chemoembolization combined with systemic vein chemotherapy in the treatment of locally advanced gastric cancer.Methods The clinical data of 78 locally advanced gastric cancer patients treated by radical D2 gastretomy from January 2010 to December 2012 were retrospectively analyzed.Patients were devided into treatment group receiving regional intra-arterial chemoembolization combined with systemic vein chemotherapy (n =36),and those with preoperative systemic vein chemotherapy (n =42) 3 to 4 weeks before surgery.Systemic chemotherapy with mFOLFOX6 was started after 5 weeks of surgery in the two groups.Results The incidence of toxicity reaction and postoperative anastomotic leakage were lower in treatment group than in control group,all P ＜ 0.05.The 1-year recurrence and overall survival rate were similar between the two groups (all P ＞ 0.05).The 3-year liver metastasis rate was higher in treatment group than in control group (x2 =4.063,P ＜ 0.05).The 3-year survival rate was higher than that of the control group(x2 =4.396,P ＜ 0.05).Conclusions Preoperative systemic vein chemotherapy combined with regional intra-arterial chemoembolization decreases toxicity reaction,anastomotic leakage and liver metastasis,while prolonging 3-year overall survival.

Effect analysis of anterior cervical operation for severe cervical kyphosis / 中华外科杂志

Objective@#To determine the feasibility and safety of anterior cervical decompression and fusion in severe cervical kyphosis treatment.@*Methods@#Totally 29 patients with severe cervical kyphosis(Cobb angle>50°) underwent anterior cervical decompression and fusion from June 2008 to May 2016 were studied retrospectively. There were 19 males and 10 females. The average age was 32.6 years ranging from 14 to 53 years. According to the etiology, 12 patients had iatrogenic deformity (11 had post-laminectomy cervical kyphosis, 1 had kyphosis due to anterior graft subsidence), 5 had neurofibromatosis, 4 had infective kyphosis, 8 had idiopathic cervical kyphosis. The curvature of cervical angle was measured by two-line Cobb method. The severity of cervical kyphosis was evaluated by kyphosis index (KI). Parameters including kyphosis levels, the apex of the kyphosis, C2-7 sagittal vertical axis(SVA) and T1 slope were also measured on lateral radiographs in the neutral position in each patient. The pre- and post-operative Japanese Orthopaedic Association(JOA) scores, visual analogue scale (VAS) of neek pain, neck disability index (NDI) and cervical alignment were compared. All patients were treated by skull traction. Motor evoked potential and somatosensory evoked potential were applied intraoperation as the spinal cord monitor.@*Results@#Skull traction was performed for an average of 6.3 days. The mean vertebral number in kyphotic region was 4.7. The average operation time was 155 minutes and blood loss was 135 ml. The preoperative C2-7Cobb angle was 46.6°±18.1° in average. It was reduced to 11.4°±6.4° in average after operation. The Cobb angle of operation region was 72.9°±19.6° in average before operation. It was reduced to 11.2°±6.4° in average after operation. The kyphosis region correction rate was 84.6%. The mean preoperative C2-7SVA changed from (3.8±14.6) mm to (12.6±7.8) mm postoperatively. The mean preoperative T1 slope changed from -10.6°±16.4° to 7.1°±14.9° postoperatively. The average postoperative C2-7 Cobb angle, Cobb angle of kyphosis region, KI, C2-7 SVA and T1 slope changed significantly compared with preoperation (F=12.700-218.200, all P<0.01). The average postoperative JOA, VAS and NDI scores improved significantly compared with preoperation (F=225.500, 217.900, 131.200, all P<0.01).@*Conclusion@#For severe cervical kyphosis, anterior correction is a safe and effective technique, sufficient decompression will be achieved.

Retrograde transposition of composite tissue flap pedicled with peroneal artery to treat bone and soft tissue defects following calcaneal osteomyelitis / 中华创伤骨科杂志

Objective To report retrograde transposition of composite tissue flap pedicled with peroneal artery in the treatment of bone and soft tissue defects following calcaneal osteomyelitis.Methods During the period from January 2008 through January 2015,47 patients with bone and soft tissue defects following calcaneal osteomyelitis were treated by retrograde transposition of composite tissue flap pedicled with peroneal artery.They were 43 men and 4 women,aged from 24 to 67 years(average,43.6 years).After radical focus debridement,the areas of skin defect ranged from 2 cm × 2 cm to 5 cm× 5 cm and the bone defects ranged from 1.5 cm × 1.0 cm × 2.0 cm to 3.0 cm × 3.0 cm × 4.5 cm.Results All the 47 patients were followed up for 6 to 72 months (average,25.2 months).Relapse of osteomyelitis occurred in 2 cases but secondary bone graft was needed in none.All the patients recovered their walking function.The texture and color of the composite tissue flaps were similar to those of normal skin,without obvious swelling.The flaps appeared good and imposed no obstacle to wearing shoes.The ankles and hind feet of the patients scored from 58 to 91 points (average,76.6 points) according to the American Orthopaedic Foot and Ankle Society (AOFAS) evaluation system,giving 7 excellent,32 good and 8 fair cases.Hallux flexion contracture without skin ulcer was observed in 10 cases 6 months after surgery,necessitating no surgical intervention.Conclusion Retrograde transposition of composite tissue flap pedicled with peroneal artery can effectively treat bone and soft tissue defects following calcaneal osteomyelitis,leading to a low recurrence rate and good foot appearance.

Isolation and Identification of Triterpenoids from Acanthopanax Sessiliflorus Fruits / 中国药师

Objective: To isolate and identify the structures of triterpenoids from the extract of Acanthopanax sessiliflorus fruits. Methods:The chemical constituents were systematically isolated by column chromatography and liquid chromatography, and their struc-tures were determined by spectral data including 1 H-NMR, 13 C-NMR, HSQC and HMBC combined with ESI-MS. Results:Totally 8 trit-erpenoids compounds were isolated and identified from Acanthopanax sessiliflorus fruits, 22-α-hydroxychiisanogenin (1), chiisanogenin (2), 3-oxo-24-methylenecycloartan (3), mangiferonic acid (4), isomangiferolic acid (5), oleanolic acid (6), betulin (7) and betulin-ic acid (8). Conclusion:Compound 3, 4 and 5 are obtained from Acanthopanax genus for the first time.

Molecular mechanisms of luteolin induced growth inhibition and apoptosis of human osteosarcoma cells

Luteolin is a flavone in medicinal plants as well as some vegetables and spices. It is a natural anti-oxidant with less pro-oxidant potential but apparently with a better safety profile. The purpose of this study was to investigate the molecular mechanism of luteolin-mediated apoptosis of MG-63 human osteosarcoma cells. MTT assay kit was employed to evaluate the effects of luteolin on MG-63 cells proliferation. Then, we performed Annexin V-FITC/PI to analyze the apoptotic rate of the cells. Furthermore, the inhibitory effects of luteolin on the expressions of BCL-2, BAX, Caspase-3 and Survivin were detected by Western blotting. As expected, luteolin [0.5, 2.5, 12.5 [microg/mL] inhibited the growth of MG-63 cells by inhibiting cell proliferation and inducing cell apoptosis. Western blotting demonstrated that luteolin [0.5, 2.5, 12.5 [microg/mL] inhibited the expressions of BCL-2, Caspase-3 and Survivin, and promoted the expression of BAX in MG-63 cells with a concentration dependent way. Luteolin can inhibit osteosarcoma cell proliferation and induce apoptosis effectively in a dose dependent manner through down-regulating the expression of BCL-2, Caspase-3 and Survivin proteins levels and up-regulating the expression of BAX protein level. These findings indicated that luteolin may be used as a novel herbal medicine for the treatment of osteosarcoma

Incidence of secondary surgical procedures after cervical disc arthroplasty compared to fusion: a meta-analysis / 中华骨科杂志

Objective To compare the incidence of secondary surgical procedures after cervical disc arthroplasty vs anterior cervical discectomy with fusion in patients treated for symptomatic single level cervical spondylosis.Methods An online search of Pubmed, Medline, Ovid, Embase, Cochrane Library, CBM database, Wanfang data and VIP database were searched for prospective randomized controlled trial of cervical disc arthroplasty versus anterior cervical discectomy with fusion in incidence of secondary surgical procedures.Data were collected and extracted by two reviewers independently.Risk of bias was assessed using the criteria of Cochrane Reviews Handbook 5.1.0.Review Manager 5.2 software system was used to evaluate the data for Meta analysis.Results A total of 12 trials were included.Six of them were short-term follow up (two years).The 6 other trials were mid-and long-term follow up (mean 5.8 years).There are 4 trials with low risk of bias, 7 trials with moderate risk of bias, and 1 trial with high risk of bias.The results of meta-analysis showed there was no statistical difference in reoperation rate of adjacent level between the two groups at two years follow-up;however, the reoperation rate of non-fusion group was significantly lower in the non-fusion group compared with fusion group at mid-and long-term follow-up.The incidence of secondary surgical procedures at index level was higher in fusion group than in non-fusion group.No statistical difference was found between the two groups in using ‘ removal’ for secondary surgical procedure at two years follow-up;however, the rate of non-fusion group was significantly lower than fusion group at mid-and long-term follow-up.Conclusion Cervical disc arthroplasty was partly superior to anterior cervical discectomy with fusion in avoiding secondary surgical procedures of adjacent levels.However, non-fusion surgery doesn't have any advantages in short time (two years) follow up.Reducing the incidence of pseudarthrosis was an effective way to decrease the incidence of secondary surgery procedures in index level.